Echelon’s Malachite Green Assay Kit is designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green, which is quantified by reading the absorbance at 620 nm. The assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast, convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green Assay kit can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2,000 pmol of phosphate per assay point. The kit includes enough reagents for 192 samples, standards, and controls.
The kit is offered in three sizes:
- K-1500: includes 2 X 96-well plates, 1 vial of phosphate standard and a bottle of malachite green solution.
- K-1520: includes 1 X K-1500 + your choice of 2 X 100 ug lipid substrates.
- K-1530: includes 1 X K-1500 + your choice of 3 X 100 ug lipid substrates.
Suggested available substrates: diC8 PI(3)P, PI(4)P, PI(5)P, PI(3,4)P2, PI(3,5)P2, PI(4,5)P2, PI(3,4,5)P3
The Malachite Green Solution can also be purchased separately.
| Phosphatase Substrate | Lipid Phosphatase Enzyme |
| PtdIns(3)P | Myotubularin, MTMR2 |
| PtdIns(4)P | |
| PtdIns(5)P | |
| PtdIns(3,4)P2 | PTEN |
| PtdIns(3,5)P2 | OCRL1, 5Ptase IV, Synaptojanin |
| PtdIns(4,5)P2 | MTMR2, PTPRQ |
| PtdIns(3,4,5)P3 | PTEN, SHIP1, SHIP2, SKIP, PTPRQ, 5ptase II |
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