CDP-MEP Synthesis Kit

Product Number: K-2000E

$717.00

$717.00
SKU: K-2000E- Category: Tags: , ,

The CDP-MEP Synthesis Kit contains the components necessary for the synthesis of CDP-MEP.

Sample Type: For Enzymatic Synthesis of CDP-MEP
Assay Incubation time: Sixty minutes (CDP-MEP Synthesis Reaction)
Sample Considerations: Yield ≥ 70% CDP-MEP (~ 0.7 mg)
Sample Volume: 1.5 mL (0.7 mg CDP-ME)

Product Background
In the fourth step of the MEP pathway, CDP-ME is converted to 4-diphosphocytidyl-2-C-methyl-D-erythritol-2-phosphate (CDP-MEP) by CDP-ME kinase (IspE) with the concomitant formation of adenosine diphosphate (ADP). The formation of CDP-MEP occurs at a 1:1 ratio with the consumption of ATP. By measuring this decrease in ATP levels the user can infer the amount of CDP-MEP generated. The user can expect 1,600 µL of >600 µM CDP-MEP produced (>60% yield).


Publications

1. Li, Z. and T. D. Sharkey (2012). “Metabolic Profiling of the Methylerythritol Phosphate Pathway Reveals the Source of Post-illumination Isoprene Burst from Leaves.” Plant, Cell & Environment.


Documents

Technical Data Sheet

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com

 

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Kits & Assays

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Isoprenoid, MEP Pathway

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