Product Number: K-3000


Echelon’s Class III PI3K ELISA Kit is a competitive (96-well) ELISA assay kit that measures Class III Phosphoinositide 3-kinases activity directly through its reaction product, PtdIns(3)P (PI(3)P).

Product Background
The Class III PI3K ELISA Kit is a competitive ELISA in which the signal is inversely proportional to the amount of PI(3)P produced. After the PI3-K reactions are complete, reaction products are added to the PI(3)P-coated microplate, for competitive binding to a PI(3)P detector protein. The amount of PI(3)P detector protein bound to the plate is determined through colorimetric detection.

Product Keywords: PI3K, PI3 Kinase, PI3 K, PI3-K, PI3P


Kits & Assays


ELISA, Kinase, PI3K

Shipping Temp

Dry ice and / or gel ice depending on destination – product may ship in two boxes


Pt 1 at 4-8 °C; Pt 2 at -20 °C

Technical Data Sheet

PI3K Assay Comparison Guide

1. Cao, X., et al. (2009). “Thyroid-hormone-dependent activation of the phosphoinositide 3-kinase/Akt cascade requires Src and enhances neuronal survival.” Biochem J 424(2): 201-209.
2. Mbengue, A., et al. (2015). “A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria.” Nature 520: 683-687.
3. Rajaram, M. V. S., et al. (2017). “M. tuberculosis Initiated Human Mannose Receptor Signaling Regulates Macrophage Recognition and Vesicle Trafficking by FcRg-Chain, Grb2, and SHP-1.” Cell Reports 21(1): 126-140.
4. Su, H., et al. (2017). “VPS34 Acetylation Controls Its Lipid Kinase Activity and the Initiation of Canonical and Non-canonical Autophagy.” Mol Cell 67(6): 907-921.
5. Singla, A., et al. (2019). “Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling.” Nature Communications 10(1): 4271.

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