Echelon’s Malachite Green Solution provides a simple and convenient method for detection of lipid and protein phosphatases. Simply mix the solution with a room temperature phosphatase reaction, incubate 15-30 min, then read (abs. 600-680nm). The liberated phosphate, released durring a phosphatase reaction, forms a colored complex with molybdate/malachite green. Malachite Green Solution can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2,000 pmol of phosphate per assay point. Each bottle contains 20 mL of solution.
Storage/stability: 6 mos. at 4°C
Please refer to the technical data sheet (TDS) for the Malachite Green Phosphatase Assay kit (K-1500) for detailed protocols.
Please also see: K-1500: Malachite Green Assay Kit