PIP Beads Sample Pack

PIP beads are composed agarose with 10 nanomoles of bound PIP per mL of beads. The sample pack consists of 0.1 mL each of all eight PIP beads and control beads. Larger quantities of control beads are also available for purchase.

Listed below are the beads contained in sample pack:
PtdIns PIP Beads
PI(3)P PIP Beads
PI(4)P PIP Beads
PI(5)P PIP Beads
PI(3,4)P2 PIP Beads
PI(3,5)P2 PIP Beads
PI(4,5)P2 PIP Bead
PI(3,4,5)P3 PIP Beads
Control Beads

Storage

4 °C – do not freeze

Applications

PIP Beads are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Publications

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1. Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Documents

Technical Data Sheet