PI(3)P Beads

Product Number: P-B003A

PI(3)P PIP Beads - Echelon Biosciences

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Lipid coated Beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein, proteins in cell lysate, or binding of radiolabeled in vitro translation products.

PI(3)P Beads are composed of agarose with 10 nanomoles of bound PI(3)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

4 °C – do not freeze

Categories	Biochemical Reagents
Filter	Beads, Phosphoinositides, PI(3)P

Technical Data Sheet

Blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Blog: Identifying lipid-protein interactions with Lipid Beads and LC/MS

1) Mosesson, Y., D. Chetrit, et al. (2009). “Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling.” Dev Cell 16(5): 687-98.
2) Zhang, P., Y. Wang, et al. (2010). “Proteomic identification of phosphatidylinositol (3,4,5) triphosphate-binding proteins in Dictyostelium discoideum.” Proceedings of the National Academy of Sciences 107(26): 11829-11834.
3) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039.

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