PI(3)P PIP Beads

Product Number: P-B003A

$133.00$691.00

$133.00
$691.00
SKU: P-B003A- Category: Tags: , , , , , , ,

PI(3)P PIP Beads are composed of agarose with 10 nanomoles of bound PI(3)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.


Applications

PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize lipid binding proteins. Possible applications include the isolation of lipid-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Storage

4 °C – do not freeze


Publications

1) Mosesson, Y., D. Chetrit, et al. (2009). “Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling.” Dev Cell 16(5): 687-98.
2) Zhang, P., Y. Wang, et al. (2010). “Proteomic identification of phosphatidylinositol (3,4,5) triphosphate-binding proteins in Dictyostelium discoideum.” Proceedings of the National Academy of Sciences 107(26): 11829-11834.
3) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. 

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”


Documents

Technical Data Sheet

We are currently moving all Documentation links to this Tab we appreciate your patience. Please feel free to contact us if you have any questions or concerns.

Categories

Biochemical Reagents

Filter

Beads, Phosphoinositides, PI(3)P

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