Phosphatidic Acid Beads are composed of agarose with 10 nanomoles of bound phosphatidic acid (PA) per 1ml of beads, enough for several protein binding experiments. Each 1 mL order of Phosphatidic Acid Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.
4 °C – do not freeze
Phosphatidic Acid Beads are designed for use in protein pull-down experiments to identify and characterize lipid binding proteins. Possible applications include the isolation of lipid-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.
Featured in Publications
1) Hakuno, D., et al. (2018). “Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction.” Scientific Reports 8(1): 16749.
2) Dudley, L. J., et al. (2019). “Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy.” The EMBO Journal: e100554.
Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”