PI(3,4,5)P3 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,4,5)P3 per mL of beads, enough for several protein binding experiment. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.
Storage
4 °C – do not freeze
Applications
PI(3,4,5)P3 PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.
Featured in Publications
1) Hiromura, M., F. Okada, et al. (2004). “Inhibition of Akt kinase activity by a peptide spanning the betaA strand of the proto-oncogene TCL1.” J Biol Chem 279(51): 53407-18.
2) Craig, H. E., J. Coadwell, et al. (2010). “ARAP3 binding to phosphatidylinositol-(3,4,5)-trisphosphate depends on N-terminal tandem PH domains and adjacent sequences.” Cellular Signalling 22(2): 257-264.
3) Takahashi, K. and K. Suzuki (2010). “WAVE2 targeting to phosphatidylinositol 3,4,5-triphosphate mediated by insulin receptor substrate p53 through a complex with WAVE2.” Cellular Signalling 22(11): 1708-1716.
4) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. http://www.bio-protocol.org/e1039
5) Barrows, D., et al. (2016). “PREX1 Protein Function is Negatively Regulated Downstream of Receptor Tyrosine Kinase Activation by p21-activated Kinases (PAKs).” The Journal of biological chemistry 291: 20042-20054.
6) Lin, A., et al. (2017). “The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.” Nat Cell Biol 19(3): 238-251.
7) Hawse, W. F. and R. T. Cattley (2019). “T cells transduce T-cell receptor signal strength by generating different phosphatidylinositols.” J Biol Chem 294(13): 4793-4805.
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