PI(3,4,5)P3 PIP Beads

Product Number: P-B345A

$133.00$691.00

$133.00
$691.00

PI(3,4,5)P3 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,4,5)P3 per mL of beads, enough for several protein binding experiment. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.


Storage

4 °C – do not freeze

Applications

PI(3,4,5)P3 PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Featured in Publications

1) Hiromura, M., F. Okada, et al. (2004). “Inhibition of Akt kinase activity by a peptide spanning the betaA strand of the proto-oncogene TCL1.” J Biol Chem 279(51): 53407-18.
2) Craig, H. E., J. Coadwell, et al. (2010). “ARAP3 binding to phosphatidylinositol-(3,4,5)-trisphosphate depends on N-terminal tandem PH domains and adjacent sequences.” Cellular Signalling 22(2): 257-264.
3) Takahashi, K. and K. Suzuki (2010). “WAVE2 targeting to phosphatidylinositol 3,4,5-triphosphate mediated by insulin receptor substrate p53 through a complex with WAVE2.” Cellular Signalling 22(11): 1708-1716.
4) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. http://www.bio-protocol.org/e1039
5) Barrows, D., et al. (2016). “PREX1 Protein Function is Negatively Regulated Downstream of Receptor Tyrosine Kinase Activation by p21-activated Kinases (PAKs).” The Journal of biological chemistry 291: 20042-20054.
6) Lin, A., et al. (2017). “The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.” Nat Cell Biol 19(3): 238-251.
7) Hawse, W. F. and R. T. Cattley (2019). “T cells transduce T-cell receptor signal strength by generating different phosphatidylinositols.” J Biol Chem 294(13): 4793-4805.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”


Documents

Technical Data Sheet

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com

 

Categories

Biochemical Reagents

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Beads, Phosphoinositides, PI(3,4,5)P3

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