PI(3,4)P2 PIP Beads

Product Number: P-B034A

$133.00$691.00

$133.00
$691.00
SKU: P-B034A- Category: Tags: , , , , , , ,

PI(3,4)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,4)P2 per mL of beads, enough for several protein binding experiment. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.


Storage

4 °C – do not freeze

Applications

PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.

Featured in Publications

1) Wullschleger, S., D. H. Wasserman, et al. (2011). “Role of TAPP1 and TAPP2 adaptor binding to PtdIns(3,4)P2 in regulating insulin sensitivity defined by knock-in analysis.” Biochemical Journal 434(2): 265-274.
2) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039.
3) Lin, A., et al. (2017). “The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.” Nat Cell Biol 19(3): 238-251.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”


Documents

Technical Data Sheet

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com

 

Categories

Biochemical Reagents

Filter

Beads, Phosphoinositides, PI(3,4)P2

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