PI(3,5)P2 Beads

Product Number: P-B035A

PI(3,5)P2 PIP Beads - Echelon Biosciences

Support

Lipid coated beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein, proteins in cell lysate, or binding of radiolabeled in vitro translation products.

PI(3,5)P2 Beads are composed of agarose with 10 nanomoles of bound PI(3,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

4 °C – do not freeze

Categories	Biochemical Reagents
Filter	Beads, Phosphoinositides, PI(3,5)P2

Technical Data Sheet

Blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Blog: Identifying lipid-protein interactions with Lipid Beads and LC/MS

1) Dong, X.-p., D. Shen, et al. (2010). “PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome.” Nat Commun 1(4): 38.
2) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039.
3) Li, X., et al. (2013). “Genetically encoded fluorescent probe to visualize intracellular phosphatidylinositol 3,5-bisphosphate localization and dynamics.” Proc Natl Acad Sci U S A 110(52): 21165-21170.

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