PI(4)P PIP Beads

Product Number: P-B004A


SKU: P-B004A- Category: Tags: , , , , , , ,

PI(4)P PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.


4 °C – do not freeze


PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.


1) Casamayor, A. and M. Snyder (2003). “Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function.” Mol Cell Biol 23(8): 2762-77.
2) Hu, Y., Z. Liu, et al. (2005). “Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings.” Proc Natl Acad Sci U S A 102(46): 16853-8.
3) Brombacher, E., S. Urwyler, et al. (2009). “Rab1 guanine nucleotide exchange factor SidM is a major phosphatidylinositol 4-phosphate-binding effector protein of Legionella pneumophila.” J Biol Chem 284(8): 4846-56.
4) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039.
5) Jiang, K., et al. (2016). “PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail.” PLoS Biol 14(2): e1002375.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”


Technical Data Sheet

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com



Biochemical Reagents


Beads, Phosphoinositides, PI(4)P

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