PI(4)P Beads

Product Number: P-B004A

PI(4)P PIP Beads - Echelon Biosciences

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Lipid coated beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein, proteins in cell lysate, or binding of radiolabeled in vitro translation products.

PI(4)P Beads are composed of agarose with 10 nanomoles of bound PI(4)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

4 °C – do not freeze

Categories	Biochemical Reagents
Filter	Beads, Phosphoinositides, PI(4)P

Technical Data Sheet

Blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Blog: Identifying lipid-protein interactions with Lipid Beads and LC/MS

1) Casamayor, A. and M. Snyder (2003). “Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function.” Mol Cell Biol 23(8): 2762-77.
2) Hu, Y., Z. Liu, et al. (2005). “Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings.” Proc Natl Acad Sci U S A 102(46): 16853-8.
3) Brombacher, E., S. Urwyler, et al. (2009). “Rab1 guanine nucleotide exchange factor SidM is a major phosphatidylinositol 4-phosphate-binding effector protein of Legionella pneumophila.” J Biol Chem 284(8): 4846-56.
4) Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039.
5) Jiang, K., et al. (2016). “PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail.” PLoS Biol 14(2): e1002375.

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