LNP Trailblazer Kits are designed for quick assembly and assessment of RNA-LNPs in biological systems. Each kit includes a buffer for preparation of RNA cargo and the necessary lipids to form lipid nanoparticles. Once formed, RNA-LNPs are stable in neutral pH buffers for several weeks allowing them to be used serially for repeat experiments. The kit components are compatible with bench top microfluidic mixers and hand mixing by pipette.
The kit is available with pre-mixed lipids in solution (K-6300M) or with individual lyophilized lipids that the user can prepare as needed (K-6300L). General protocols for preparing RNA-LNPs based on a standard eGFP mRNA are included, however optimization for each individual RNA or oligonucleotide should be done by the end user.