The PIP3 Mass ELISA detects PI(3,4,5)P3 (PIP3), extracted from cells, by means of a competitive 96-well ELISA assay.
Assay Range: 125 to 0.04 pmol PIP3
Sample Type: Dried down lipid extracted from cells
Sample Volume: 60 µL/sample, single point
The PIP3 Mass ELISA Kit is a competitive ELISA designed for the sensitive detection of PIP3 from cell samples.
For detection of PIP3, PIP3 is extracted from millions of cells and incubated with a specialized PIP3 detector protein. This mixture is then added to a PIP3-coated microplate, where competitive binding occurs. Detection is achieved using a peroxidase-linked secondary reagent along with a colorimetric substrate. The resulting colorimetric signal inversely correlates with the amount of PIP3 extracted from the samples.
PIP3 production by class I PI3-kinases (PI3-K) plays a crucial role in various cell signaling pathways. Traditionally, measuring PI3-K activity involved labor-intensive methods using 32P-labeled substrates and thin-layer chromatography. Our PIP3 Mass ELISA Kit simplifies this process, allowing researchers to accurately determine PI3-K activity through a standard ELISA format—eliminating the need for radioactivity and chromatography.
Keywords: Phosphatidylinositol (3,4,5)-trisphosphate, PtdIns(3,4,5)P3, PI(3,4,5)P3, PIP3, Phosphatidylinositol 3-kinase, PI 3-kinase, PI3-Kinase, PI3-K, PI3K, PI3 Kinase, PI3 K, cell extraction, lipid extraction
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