Control PolyPIPosomes

Product Number: Y-0000

PolyPIPosomes - Echelon Biosciences

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Product Description

PolyPIPosomes are polymerized, liposome-like nanoparticles which show markedly increased stability (up to 6 months) vs. conventional liposomes. A biotin tag is incorporated for easy detection of PIP_n-binding proteins with streptavidin reagents or coated surfaces.
PolyPIPosomes have demonstrated utility in a number of assay formats:

-Protein pull-down
-Surface Plasmon Resonance
-Liposome overlay assay

Control PolyPIPosomes are provided as a 1mM total lipid solution without any PIP_n for use as a negative control to confirm there is no non-specific binding.

Additional Information

Categories	Biochemical Reagents
Filter	Phosphoinositides, PolyPIPosomes
Storage	4 °C. Do not freeze!

Technical Data Sheet

1) Ferguson, C. G., R. D. James, et al. (2005). “Phosphoinositide-Containing Polymerized Liposomes: Stable Membrane-Mimetic Vesicles for Protein-Lipid Binding Analysis.” Bioconjug Chem 16(6): 1475-1483.
2) Elkin, S. K., D. Ivanov, et al. (2005). “A PHD finger motif in the C-terminus of RAG2 modulates recombination activity.” J Biol Chem 280(31): 28701-10.
3) Mustafa, A. K., D. B. van Rossum, et al. (2009). “Glutamatergic regulation of serine racemase via reversal of PIP2 inhibition.” Proc Natl Acad Sci U S A 106(8): 2921-6.
4) Zemskov, E. A., I. Mikhailenko, et al. (2011). “Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.” PLoS One 6(4): e19414.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”