The Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate.
Assay sensitivity: 25 pmol
Sample Type: tissue homogenates, cell lysates
Sample Volume: 50 µL/well
The Acid Sphingomyelinase Assay Kit utilizes a fluorogenic substrate, that is specific for acid sphingomyelinase, to provide a sensitive and homogenous method to measure the activity of aSMase in vitro. The kit provides all necessary reagents to measure the acid sphingomyelinase activity of 40 samples ran in duplicate.
Sphingomyelinases catalyzes the hydrolysis of sphingomyelin into ceramide and phosphoryl choline; and is involved in programmed cell death (apoptosis), cell differentiation and cell proliferation. Sphingomyelinases are classified into five categories: acid sphingomyelinase (aSMase), secretory sphingomyelinase (sSMase), neutral Mg 2 +/- dependent sphingomyelinases (nSMase), neutral Mg 2 +/- independent sphingomyelinases and alkaline sphingomyelinase. Acid sphingomyelinase was the first described and best characterized of the sphingomyelinases. A deficiency of lysosomal acid sphingomyelinase leads to rapid neurodegeneration and death due to excessive accumulation of sphingomyelin (Niemann-Pick disease).