Acid Sphingomyelinase Activity Assay Kit

Product Number: K-3200


The Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate.

Assay sensitivity: 25 pmol
Sample Type: tissue homogenates, cell lysates
Sample Volume: 50 µL/well

Product Background
The  Acid Sphingomyelinase Assay Kit utilizes a fluorogenic substrate, that is specific for acid sphingomyelinase, to provide a sensitive and homogenous method to measure the activity of aSMase in vitro. The kit provides all necessary reagents to measure the acid sphingomyelinase activity of 40 samples ran in duplicate.

Sphingomyelinases catalyzes the hydrolysis of sphingomyelin into ceramide and phosphoryl choline; and is involved in programmed cell death (apoptosis), cell differentiation and cell proliferation. Sphingomyelinases are classified into five categories: acid sphingomyelinase (aSMase), secretory sphingomyelinase (sSMase), neutral Mg 2 +/- dependent sphingomyelinases (nSMase), neutral Mg 2 +/- independent sphingomyelinases and alkaline sphingomyelinase. Acid sphingomyelinase was the first described and best characterized of the sphingomyelinases. A deficiency of lysosomal acid sphingomyelinase leads to rapid neurodegeneration and death due to excessive accumulation of sphingomyelin (Niemann-Pick disease).


Kits & Assays


Activity, Enzyme

Shipping Temp

Dry ice or gel ice depending on destination


-20 °C

Technical Data Sheet

1. Li, J., W. Yu, et al. (2010). “alpha-TEA-induced death receptor dependent apoptosis involves activation of acid sphingomyelinase and elevated ceramide-enriched cell surface membranes.” Cancer Cell Int 10: 40
2. Lopes Pinheiro, M. A., et al. (2016). “Acid Sphingomyelinase-Derived Ceramide Regulates ICAM-1 Function during T Cell Transmigration across Brain Endothelial Cells.” J Immunol 196(1): 72-79.
3. Novgorodov, S. A., et al. (2017). “Acid sphingomyelinase promotes mitochondrial dysfunction due to glutamate-induced regulated necrosis.” Journal of Lipid Research 59: 312-329.
4. Novgorodov, S. A., et al. (2019). “Acid sphingomyelinase deficiency protects mitochondria and improves function recovery after brain injury.” Journal of Lipid Research 60: 609-623.
5. Yeganeh, B., et al. (2019). “Acid Sphingomyelinase Inhibition Attenuates Cell Death in Mechanically-Ventilated Newborn Rat Lung.” Am J Respir Crit Care Med 199(6): 760-772.

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