Autotaxin Activity Assay

Product Number: K-4100


The Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.

Sample Volume: 400 µL / sample (50 µL per well, 8 wells per sample)
Sample Number: 10 samples (in duplicate) / 96 well assay
Sample Type: Currently this kit has been tested for the quantification of autotaxin activity in serum, plasma, ovarian ascites, and cell culture media.

Product Background
The Autotaxin Activity Assay, developed by Echelon Biosciences Inc., utilizes the fluorogenic autotaxin substrate, FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence however, once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.

This assay gives a quantitative measurement of autotaxin activity in biological samples by measuring changes in fluorescence over time. Because the assay is not end point based, it has the advantage of being less susceptible to error arising from mistiming the addition of components to different sample wells. The final readout is in quantitative units that correct for inconsistencies between different users equipment.

Note to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. For inquiries email

Product Keywords: ATX, LPA, FS-3, LPC, lysophospholipase D


Kits & Assays


Activity, Autotaxin

Shipping Temp

Dry ice or gel ice depending on destination


-20 °C

Technical Data Sheet

1. Gaetano, C. G., N. Samadi, et al. (2009). “Inhibition of autotaxin production or activity blocks lysophosphatidylcholine-induced migration of human breast cancer and melanoma cells.” Molecular Carcinogenesis 48(9): 801.
2. Braeuer, R. R., M. Zigler, et al. (2012). “Galectin-3 Contributes to Melanoma Growth and Metastasis via Regulation of NFAT1 and Autotaxin.” Cancer Research 72(22): 5757-5766.
3. Shelton, E. L., C. L. Galindo, et al. (2013). “Autotaxin Signaling Governs Phenotypic Heterogeneity in Visceral and Parietal Mesothelia.” PLoS ONE 8(7): e69712.
4. Nsaibia, M. J., et al. (2016). “Autotaxin interacts with lipoprotein(a) and oxidized phospholipids in predicting the risk of calcific aortic valve stenosis in patients with coronary artery disease.” Journal of Internal Medicine 280: 509-517.
5. Cochrane, W., et al. (2019). “Activity-Based DNA-Encoded Library Screening.” ACS Combinatorial Science 21(5): 425-435.

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