CDP-MEP Synthesis Kit

Catalog No.:  K-2000E

Description:  In the fourth step of the MEP pathway, CDP-ME is converted to 4-diphosphocytidyl-2-C-methyl-D-erythritol-2-phosphate (CDP-MEP) by CDP-ME kinase (IspE) with the concomitant formation of adenosine diphosphate (ADP). The formation of CDP-MEP occurs at a 1:1 ratio with the consumption of ATP. By measuring this decrease in ATP levels the user can infer the amount of CDP-MEP generated. The user can expect 1,600 µL of >600 µM CDP-MEP produced (>60% yield).



Featured in Publications

1. Li, Z. and T. D. Sharkey (2012). "Metabolic Profiling of the Methylerythritol Phosphate Pathway Reveals the Source of Post-illumination Isoprene Burst from Leaves." Plant, Cell & Environment.

Related Products

MEP Pathway Reagents
MEP Pathway inhibitors: FR900098 (K-4202), 5-Ketoclomazone (K-4101)
CDP-ME Synthase (IspD) Enzymatic Assay (K-2000D)
MEP Pathway Enzymes: DXP Synthase (E-20DXS), MEP Synthase (E-2000C), IspD Enzyme (E-2000D), IspE Enzyme (E-2000E), IspF Enzyme

Options/Sizes Pricing
K-2000E-1kit $ 631.00
Additional Information:
Tech Data Sheet - TDS_K-2000D_Rev5

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assay and reagents for drug discovery in lipid signaling pathways