Nanobody Production Service

Nanobodies are single domain antibodies produced from camelids by immunization with a target antigen, similar to the process used in mice and rabbits for traditional antibody generation. However, because of the single heavy chain found in camelid antibodies, cDNAs can be generated from this single domain which can then be expressed recombinantly. This opens up a wide variety of applications that are not tenable with traditional IgG, IgM, or Fab antibodies. 

Why make a Nanobody?

Nanobodies have two main advantages over conventional antibodies: 1) their small size and 2) recombinant expression

The cloned camelid heavy chain that gives rise to a nanobody (VHH antibody) is approximately 15 kDa in size. This is far smaller than the 150 kDa IgG antibodies that are employed for most research. With the availability of the cDNA, this means nanobodies can be expressed or transduced in cells. This creates the possibility of tracking a protein of interest without having to create a GFP-fusion protein or even disrupt protein-protein interactions without the need for RNAi or knockouts. 

The single domain structure of nanobodies also means they are much more stable across a range of conditions that would be restrictive to traditional antibodies. This opens up the possibility of using them as scaffolds or epitope mapping tools in protein crystallization, NMR, and CryoEM. 

Possible Applications:

  • Add an affinity tag for pulldown and purification experiments
  • Conjugate fluorophores for super-resolution microscopy
  • Use them as inhibitors to bind active site clefts
  • Targeted protein degradation similar to PROTACs

Our services

Immunization can proceed with a purified antigen provided by you, or we can generate this in-house. We will guide you through the entire process with regular reports submitted after each major step. The entire production time is typically 6-8 weeks from immunization.

Quotes will be generated according to service type and for specific project needs. We can produce nanobodies starting with full immunization or beginning from a known target sequence. Contact us using the form below to get started on your nanobody project.

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