Anti-hVps34 Antibody for IP

Z-R015 is an affinity purified rabbit polyclonal anti-hVps34 antibody.  It can immunoprecipicate human Vps34 from cell lysate without inhibition.  There is no need to elute the enzyme prior to running and activity assay.  See figure above for hVps34 activity data obtained using Z-R015 in IP. 

Antigen: human Vps34 (PIK3C3, Class III PI3-Kinase). 

For Western Blot application and immunostaining, we recommend anti-hVps34 antibody, cat# Z-R016. 

Concentration

250 µg/mL

Formulation

Antibody is formulated in 100 mM Tris-HCl, 100 mM glycine, pH 7.5, and 50% glycerol.

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Publications

1. Singla A, Fedoseienko A, Giridharan SSP, Overlee BL, Lopez A, Jia D, et al. (2019) Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling. Nature Communications.
2. Scotto Rosato A, Montefusco S, Soldati C, Di Paola S, Capuozzo A, Monfregola J, et al. (2019) TRPML1 links lysosomal calcium to autophagosome biogenesis through the activation of the CaMKKβ/VPS34 pathway. Nature Communications.
3. Su H, Yang F, Wang Q, Shen Q, Huang J, Peng C, et al. (2017) VPS34 Acetylation Controls Its Lipid Kinase Activity and the Initiation of Canonical and Non-canonical Autophagy. Mol Cell.