While once thought of as a passive scaffold for cells, there is a rapidly growing appreciation for the extracellular matrix (ECM) as a physiologically active space. Chief among ECM components is Hyaluronic Acid (HA) which participates in cell adhesion and cell-to-cell communication and has been implicated in the development of cancer and cardiovascular disease. Echelon Biosciences has developed a number of tools to interrogate HA function, including ELISAs, fluorescent conjugates, and medical grade reagents.
The Hyaluronidase Activity ELISA is a quantitative immunoassay designed for in vitro measurement of hyaluronidase activity in biological samples.
Select-HA HiLadder is a HA preparation of uniform and narrow size distribution prepared by in vitro synthesis using recombinant Pasteurella multocida hyaluronan synthase.
Echelon’s Biotinylated Versican G1 Domain (biotin VG1) contains only the G1 domain from versican, an HA binding protein. It is suitable for visualizing HA via immunostaining in cells.
Competitive HA ELISA
Echelon Biosciences offers Hyaluronic Acid ELISAs in two different formats. The Competitive HA ELISA is designed for the detection and measurement of HA from biological fluids including blood, serum, and urine. The simple protocol requires less than three hours to complete and is independent of the molecular weight of HA species. The assay is sensitive to HA polymers of 25 units and larger.
Hyaluronidase Activity ELISA
Hyaluronidases are a group of enzymes that degrade HA. Hyaluronidases have become an important area of study due to their regulatory function in HA metabolism and research has also shown that hyaluronidases are involved in several pathological processes such as bacterial pathogenesis, the spreading of toxins/venoms and cancer progression, making them a potential pharmacological target.
Echelon Biosciences is now the sole manufacturer and distributor for Select-HA™ products originally developed by Hyalose, LLC. Unlike other commercial hyaluronic acid, which have much greater size heterogeneity, each Select-HA™ runs as a tight band with discrete size. With low endotoxin level, individual Select-HA™ reagents are also suitable for cell culture experiments.