Molecular Weight: 720.28
Salt Form: TFA
Sequence (3-letter): Z-Tyr-Val-Ala-Asp-pNA
Sequence (1-letter): Z-YVAD-pNA
Storage: -20 °C or below
Z-Tyr-Val-Ala-Asp-pNA is a colorimetric substrate for Caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that preferentially cleaves substrates built on VAD (val-ala-asp) or WEAD (trp-glu-ala-asp) sequences. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cells.
Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.