Sphingo Strips – Lipid-Protein Interaction Assay

Product Number: S-6000

$183
$522

The original lipid-protein interaction assay and the trusted solution for the identification of protein-lipid interactions.

  • Determine the binding specificity of lipid-binding proteins
  • Includes 15 major sphingolipids
  • 20+ years’ experience, proven & published
  • Cost effective 10-pack

A simple and effective tool designed for the screening and analysis of protein-lipid interactions. Sphingo Strips, a 2 x 6 cm membrane, contain 15 sphingolipids including ceramide, sphingosine, lysophosphatidic acid, and a blue blank control. Sphingolipids are a class of lipids containing a backbone of sphingoid bases. They play a crucial roles in both structural integrity and cell signaling.

Simple to use, once the membrane is blocked and incubated with the protein of interest, the membrane is washed. Then incubated with an HRP labeled secondary antibody. The membrane is then washed before detection with precipitating TMB or ECL reagent.

Each batch of Sphingo Strips undergoes testing against a panel of published lipid-binding proteins, ensuring consistent quality and reliability. Echelon offers a wide array of lipid strips and arrays. Find the one that works best for your research.

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Kits & Assays

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Sphingolipid, Lipid Strips and Arrays

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Ambient Temperature

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4-8 °C, protect from light

Technical Data Sheet

Protocol

FAQ

Blog: 5 tips to improve your strips!

1) Andersson, E., F. Schain, et al. (2006). “Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity.” Biochim Biophys Acta 1761(12): 1498-505.
2) Krishnamurthy, K., S. Dasgupta, et al. (2007). “Development and characterization of a novel anti-ceramide antibody.” J Lipid Res 48(4): 968-75.
3) Poon, I. K., M. D. Hulett, et al. (2010). “Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcgammaRI on phagocytes.” Blood 115(12): 2473-82.

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