Sphingo Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of fifteen different sphingolipids. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.
Sphingo Strips contain the following lipids at 100 pmol per spot: (click picture above for exact layout)
Sphingosine 1-phosphate (S1P)
Lysophosphatidic Acid (LPA)
2-8 °C. Product is moisture and light sensitive
Featured in Publications
1) Andersson, E., F. Schain, et al. (2006). “Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity.” Biochim Biophys Acta 1761(12): 1498-505.
2) Krishnamurthy, K., S. Dasgupta, et al. (2007). “Development and characterization of a novel anti-ceramide antibody.” J Lipid Res 48(4): 968-75.
3) Poon, I. K., M. D. Hulett, et al. (2010). “Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcgammaRI on phagocytes.” Blood 115(12): 2473-82.
Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”