Sphingo Strips – Lipid-Protein Interaction Assay

Product Number: S-6000

$174.00$497.00

$174.00
$497.00
SKU: S-6000- Category: Tags: , , , , ,

Sphingo Strips are membranes pre-spotted with 15 different sphingolipids and blank control.  Sphingo Strips are used in a simple protein-lipid overlay assay to help determine the specificity of your protein towards one or more of these lipids.

Briefly, once the membrane is blocked, it is incubated with your protein of interest.  The membrane is then washed and incubated with an HRP labeled antibody against the protein or your proteins affinity tag. The membrane is washed again before incubation with ECL or a precipitating TMB detection reagent. This can then be visualized with the appropriate equipment based on the detection reagent used.

Sphingo Strips are 2 x 6 cm hydrophobic membranes with 100 pmol of the following lipids: Sphingosine, Sphingosine 1-phosphate (S1P), Phytosphingosine, Ceramide, Sphingomyelin, Sphingosylphosphorylcholine (SPC), Lysophosphatidic Acid (LPA), Myriosine, Monosialoganglioside-GM1, Disialoganglioside-GD3, Sulfatide, Psychosine, Cholesterol, Lysophosphatidylcholine (LPC), Phosphatidylcholine (PC).

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Being bio-reactive blog:“Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Blog: 5 tips to improve your strips!

1) Andersson, E., F. Schain, et al. (2006). “Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity.” Biochim Biophys Acta 1761(12): 1498-505.
2) Krishnamurthy, K., S. Dasgupta, et al. (2007). “Development and characterization of a novel anti-ceramide antibody.” J Lipid Res 48(4): 968-75.
3) Poon, I. K., M. D. Hulett, et al. (2010). “Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcgammaRI on phagocytes.” Blood 115(12): 2473-82.

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