PIP Strips are membranes pre-spotted with all eight phosphoinositides, seven other biological important lipids, and a blank control. PIP Strips are used in a simple protein-lipid overlay assay to help determine the specificity of your protein towards one or more of these lipids.
Briefly, once the membrane is blocked, it is incubated with your protein of interest. The membrane is then washed and incubated with an HRP labeled antibody against the protein or your proteins affinity tag. The membrane is washed again before incubation with ECL or a precipitating TMB detection reagent. This can then be visualized with the appropriate equipment based on the detection reagent used.
PIP Strips are 2 x 6 cm hydrophobic membranes with 100 pmol of the following lipids: Lysophosphatidic acid (LPA), Lysophosphocholine (LPC), Phosphatidylinositol (PtdIns), Phosphatidylinositol (3)-phosphate (PtdIns(3)P), Phosphatidylinositol (4)-phosphate (PtdIns(4)P), Phosphatidylinositol (5)-phosphate (PtdIns(5)P), Phosphatidylethanolamine (PE), Phosphatidylcholine (PC), Sphingosine 1-Phosphate (S1P), Phosphatidylinositol (3,4)-bisphosphate (PtdIns(3,4)P2 ), Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2), Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2), Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3), Phosphatidic acid (PA), Phosphatidylserine (PS).
Each 10-pack of PIP Strips includes a free vial of the PI(4,5)P2 specific binding protein as a positive control. For a list other phosphoinositide binding proteins, click here.
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