PIP Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of all eight phosphoinositides and seven other biological important lipids. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.
PIP Strips contain the following lipids at 100 pmol per spot: (click picture above for exact layout)
Lysophosphatidic acid (LPA)
Lysophosphocholine (LPC)
Phosphatidylinositol (PtdIns)
Phosphatidylinositol (3)-phosphate (PtdIns(3)P)
Phosphatidylinositol (4)-phosphate (PtdIns(4)P)
Phosphatidylinositol (5)-phosphate (PtdIns(5)P)
Phosphatidylethanolamine (PE)
Phosphatidylcholine (PC)
Sphingosine 1-Phosphate (S1P)
Phosphatidylinositol (3,4)-bisphosphate (PtdIns(3,4)P2 )
Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2)
Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2)
Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3)
Phosphatidic acid (PA)
Phosphatidylserine (PS)
Each 10-pack of PIP Strips includes a free vial of the PI(4,5)P2 specific binding protein for use as a positive control. For a list other phosphoinositide binding proteins, click here.
Storage
2-8 °C. Product is moisture and light sensitive
Publications
Powered by Bioz1) Huang, S., L. Gao, et al. (2006). “Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid.” Molecular Biology of the Cell 17(4): 1946-1958.
2) Jin, W., W.-P. Ge, et al. (2006). “Lipid Binding Regulates Synaptic Targeting of PICK1, AMPA Receptor Trafficking, and Synaptic Plasticity.” The Journal of Neuroscience 26(9): 2380-2390.
3) Xi, Q., G. J. T. Pauer, et al. (2005). “Tubby-like Protein 1 (TULP1) Interacts with F-actin in Photoreceptor Cells.” Investigative Ophthalmology & Visual Science 46(12): 4754-4761.
4) Sharma, V. P., V. Desmarais, et al. (2008). “Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells.” J Leukoc Biol 84(2): 440-7.
Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”
