PIP Strips – Lipid-Protein Interaction Assay

Product Number: P-6001


PIP Strips are membranes pre-spotted with all eight phosphoinositides, seven other biological important lipids, and a blank control.  PIP Strips are used in a simple protein-lipid overlay assay to help determine the specificity of your protein towards one or more of these lipids.

Briefly, once the membrane is blocked, it is incubated with your protein of interest.  The membrane is then washed and incubated with an HRP labeled antibody against the protein or your proteins affinity tag. The membrane is washed again before incubation with ECL or a precipitating TMB detection reagent. This can then be visualized with the appropriate equipment based on the detection reagent used.

PIP Strips are 2 x 6 cm hydrophobic membranes with 100 pmol of the following lipids: Lysophosphatidic acid (LPA), Lysophosphocholine (LPC), Phosphatidylinositol (PtdIns), Phosphatidylinositol (3)-phosphate (PtdIns(3)P), Phosphatidylinositol (4)-phosphate (PtdIns(4)P), Phosphatidylinositol (5)-phosphate (PtdIns(5)P), Phosphatidylethanolamine (PE), Phosphatidylcholine (PC), Sphingosine 1-Phosphate (S1P), Phosphatidylinositol (3,4)-bisphosphate (PtdIns(3,4)P2 ), Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2), Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2), Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3), Phosphatidic acid (PA), Phosphatidylserine (PS).

Each 10-pack of PIP Strips includes a free vial of the PI(4,5)P2 specific binding protein as a positive control. For a list other phosphoinositide binding proteins, click here.


Kits & Assays


Phosphoinositides, Strip

Technical Data Sheet



Being bio-reactive blog:“Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Blog: 5 tips to improve your strips!

1) Huang, S., L. Gao, et al. (2006). “Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid.” Molecular Biology of the Cell 17(4): 1946-1958.
2) Jin, W., W.-P. Ge, et al. (2006). “Lipid Binding Regulates Synaptic Targeting of PICK1, AMPA Receptor Trafficking, and Synaptic Plasticity.” The Journal of Neuroscience 26(9): 2380-2390.
3) Xi, Q., G. J. T. Pauer, et al. (2005). “Tubby-like Protein 1 (TULP1) Interacts with F-actin in Photoreceptor Cells.” Investigative Ophthalmology & Visual Science 46(12): 4754-4761.
4) Sharma, V. P., V. Desmarais, et al. (2008). “Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells.” J Leukoc Biol 84(2): 440-7.

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