PIP Arrays – Lipid-Protein Interaction Assay

Product Number: P-6100

$340
$626

The original lipid-protein interaction assay and the trusted solution for the identification of protein-lipid interactions.

  • Determine the binding specificity and sensitivity of lipid-binding proteins
  • Includes 8 phosphoinositides at 7 concentrations
  • 20 + years’ experience, proven & well-published
  • Cost effective 5-pack includes a complimentary vial of positive control

A simple and effective tool designed for the screening and analysis of protein-lipid interactions. PIP Arrays, a 4 x 5 cm membrane, contain 8 phosphoinositides at 7 concentrations. Phosphoinositides are a family of acidic phospholipids found in cell membranes. They interact with proteins and contribute to the regulation of cellular processes.

Simple to use, once the membrane is blocked and incubated with the protein of interest, the membrane is washed. Then incubated with an HRP labeled secondary antibody. The membrane is then washed before detection with precipitating TMB or ECL reagent.

Each batch of PIP Arrays undergoes rigorous testing against a diverse panel of published lipid-binding proteins, ensuring consistent quality and reliability. Echelon offers a wide array of lipid strips and arrays. Find the one that works best for your research. Explore additional lipid binding proteins.

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Kits & Assays

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Phosphoinositides, Lipid Strips and Arrays

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Ambient Temperature

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4-8 °C, protect from light

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Blog: 5 tips to improve your strips!

1) Gozani, O., P. Karuman, et al. (2003). “The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor.” Cell 114(1): 99-111.
2) Lindsay, A. J. and M. W. McCaffrey (2004). “The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane.” J Cell Sci 117(19): 4365-4375.
3) Andersson, E., F. Schain, et al. (2006). “Interaction of human 15-lipoxygenase-1 with phosphatidylinositol bisphosphates results in increased enzyme activity.” Biochim Biophys Acta 1761(12): 1498-505.
4) Guittard, G., A. Gerard, et al. (2009). “Cutting Edge: Dok-1 and Dok-2 Adaptor Molecules Are Regulated by Phosphatidylinositol 5-Phosphate Production in T Cells.” J Immunol 182(7): 3974-3978.

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