Full-length recombinant human PI4KIIA was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Enzyme obtained from SignalChem.
Molecular weight: ~79 kDa
Sequence: Full length
Tag: GST tag
Purity: >90 % (request TDS for lot specific information)
Specific Activity: >30 nmol/min/mg (request TDS for lot specific information)
Expression System: Sf9 insect cells using baculovirus
Genebank Number: NM_018425
Applications: PI 4-Kinase assay, western blot.
Formulation: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Storage and stability: Store product at -70ºC. Stable up to one year.
PI4KIIA or phosphatidylinositol 4-kinase type 2 alpha is involved in the formation of phosphatidylinositolpolyphosphates (PtdInsPs) which are centrally involved in many biologic processes ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KIIA phosphorylates PtdIns at the D-4 position which is an essential step in the biosynthesis of PtdInsPs. PI4KIIA is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation. PI4KIIA generates PtdIns4P-rich domains within the Golgi which regulate targeting of clathrin adaptor AP-1 complexes. PI4KIIA is also required for Wnt to regulate LRP6 phosphorylation.