The High Throughput Autotaxin Inhibitor Screening Kit uses purified autotaxin (ATX) and the autotaxin substrate FS-3, which fluoresces upon cleavage. The assay is performed in a 384 well plate and quantifies the autotaxin activity in the presence of the user’s samples by measuring changes in fluorescence over time.
The High Throughput Autotaxin Inhibitor Screening Kit, developed by Echelon Biosciences Inc., uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.
Percent autotaxin inhibition is determined by referencing no inhibitor controls. The autotaxin inhibitor, BrP-LPA, is supplied to positively control for autotaxin inhibition. The assay is resistant to modest concentrations of DMSO and is performed in a continuous assay format, which has the advantage of being less susceptible to error arising from mis-timing the addition of components to different sample wells.
Product Keywords: ATX, LPA, FS-3, LPC, lysophospholipase D, BrP-LPA
Note to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. For inquiries email firstname.lastname@example.org