Autotaxin Inhibitor Screening Kit

Product Number: K-4200

$537

The Autotaxin Inhibitor Screening Kit utilizes purified autotaxin (ATX) and the autotaxin substrate FS-3, which fluoresces upon cleavage. The assay is performed in a 96 well plate and quantifies the autotaxin activity in the presence of the user’s samples by measuring changes in fluorescence over time.


Sample Type: Inhibitors against ATX
Sample Volume: 10 µL/ well of your sample compounds
Sample Considerations: Some organic solvents interfere with the assay (see technical data sheet).


Product Background
The Autotaxin Inhibitor Screening Kit, developed by Echelon Biosciences Inc., uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence however, once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.

Percent autotaxin inhibition is determined by referencing no inhibitor controls. The autotaxin inhibitor, BrP-LPA, is supplied to positively control for autotaxin inhibition. The assay is resistant to modest concentrations of DMSO and is performed in a continuous assay format, which has the advantage of being less susceptible to error arising from mis-timing the addition of components to different sample wells.

Product Keywords: ATX, LPA, FS-3, LPC, lysophospholipase D, BrP-LPA

Note to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. For inquiries email busdev@echelon-inc.com

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Kits & Assays

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Autotaxin, Inhibitor

Shipping Temp

Dry ice or gel ice depending on destination

Storage

-20 °C

Technical Data Sheet

1. Altman, M.K., Gopal, V., Jia, W., Yu, S., Hall, H., Mills, G.B., McGinnis, A.C., Bartlett, M.G., Jiang, G., Madan, D., Prestwich, G.D., Xu, Y., Davies, M.A., & Murph, M.M., Targeting melanoma growth and viability reveals dualistic functionality of the phosphonothionate analogue of carba cyclic phosphatidic acid. 2010. Mol Cancer 9, 140.
2. Jiang, G., Madan, D., & Prestwich, G.D., Aromatic phosphonates inhibit the lysophospholipase D activity of autotaxin. Bioorg Med Chem Lett.

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