Use of this kinase reaction buffer improves Class I PI3-Kinase activities for all isoforms. The buffer is compatible with all of our PI3K activity assays and substrates and is already included with the K-1000s kit. Each vial contains 4 mL of 5X buffer. Please refer to the K-1000s Technical Data Sheet for further details and procedures.
Class I Phosphoinositide 3-kinases (PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PI(4,5)P2 at the 3′-hydroxyl of the inositol ring producing PtdIns(3,4,5)P3. PtdIns(3,4,5)P3 has shown to be an important lipid second messenger with key roles in fundamental cellular responses such as proliferation and survival. Due to these interesting biological roles, PI3-K and PtdIns(3,4,5)P3 have become attractive targets for research and drug development in many diseases including inflammation and cancer. Echelon has multiple formats available for the determination of class I PI3-K activity.