PI(3,4)P2 Mass ELISA

The PI(3,4)P2 Mass ELISA measures the amount of PI(3,4)P2 extracted from cells by means of a competitive ELISA (96 well format).

Assay Range: 300 to 1.23 pmol PI(3,4)P2
Sample Type: Dried down lipid extracted from cells
Sample Volume: 120 µL/sample, run in duplicate.

The PI(3,4)P2 Mass ELISA is a competitive ELISA where the signal is inversely proportional to the amount of PI(3,4)P₂ produced. This allows the user to measure the amount of PI(3,4)P₂ extracted from cells by means of standard 96 well ELISA format. Eliminating the need for radioactivity and thin layer chromatography.

Once PI(3,4)P₂ has been extracted from cellular samples, it is incubated with a PI(3,4)P₂ detector, then added to the PI(3,4)P₂-coated plate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(3,4)P₂ detector binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4)P₂ extracted from cells.

Keywords: PI(3,4)P2, PI34P2, PtdIns(3,4)P2

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