Molecular Weight: 760.2
Salt Form: N/A
Sequence (3-letter): Ac-Asp-Gln-Met-Asp-AFC
Sequence (1-letter): Ac-DQMD-AFC
Storage: -20 °C or below
Ac-Asp-Gln-Met-Asp-AFC is a fluorogenic substrate for caspase 3. Caspase 3 is a cysteine protease that is rapidly activated during apoptosis and cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Caspase 3 is activated by Caspases 8, 9 and 10 and cleaves and activates caspase 6 and 7. Caspase 3 cleaves the amyloid precursor protein (APP) contributing to Amyloid beta formation and has been implicated in Alzheimer’s disease and proposed as a target for drug discovery.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.