CAS Number: 76524-84-0
Molecular Weight: 1299.66
Salt Form: TFA
Purity: >96%
Sequence (3-letter): Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-AMC
Sequence (1-letter): Suc-RPFHLLVY-AMC
Storage: -20 °C or below
Renin Substrate I is a fluorogenic substrate for Renin which controls vasoconstriction and blood pressure as part of the Renin-angiotensin system (RAS). This peptide also acts as a substrate for the chymotrypsin-like activity of the 20S proteasome.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.
References
- K. Murakami, T. Ohsawa, S. Hirose, K. Takada, and S. Sakakibara, Anal. Biochem., 110, 232 (1981).
- H. Yokosawa, H. Ito, S. Murata, and S. Ishii, Anal. Biochem., 134, 210 (1983).
