The Sphingosine 1-Phosphate ELISA kit (S1P ELISA) is a sensitive and robust method for the quantification of S1P in biological samples (96-well format).
Assay Range: 0.0625 µM – 2 µM
Sample Volume: For duplicate data points 25 µL of each sample is required.
Sample Type: This kit detects S1P from human or animal in serum, plasma, tissue homogenate, and cell lysate, This assay is not species specific and has been tested with S1P from human, bovine, equine, caprine, and mouse.
Product Background
The Sphingosine 1-Phosphate ELISA kit (S1P ELISA) measures S1P in biological samples.
Assay Overview
To run the S1P ELISA, the S1P coated plate is first blocked to reduce non-specific binding. Then the S1P standards and samples are combined with an anti-S1P antibody before it is transferred to the blocked S1P coated plate. The S1P coated plate and the S1P in the sample compete for binding of the anti-S1P antibody during this step. Following an incubation and plate wash, streptavidin-HRP is added to the plate for detection of any bound anti-S1P antibodies (labeled with biotin). After an additional incubation and plate wash, TMB substrate is added to the plate. The colorimetric TMB reaction is stopped with the addition of 1N sulfuric acid and the plate is measured at 450 nm absorbance. The concentration of S1P, in the samples, is determined when compared to the S1P standard curve.
Scientific Significance
Sphingosine 1-Phosphate (S1P) is a bioactive lipid involved in the sphingolipid signaling cascade. S1P drives cell growth, blood vessel formation, and resistance to cell death—key factors in cancer progression. Emerging research highlights S1P as a potential tumorigenic growth factor and proposes its utility as an early-stage cancer biomarker. Additionally, Sphingosine kinase, the enzyme responsible for converting sphingosine to S1P, has been shown to be up regulated in a variety of cancer types.
S1P Lyase Activity Detection
A modified protocol allows for the detection of S1P lyase activity using this kit is under the documentation tab.
Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit is covered by a U.S. Patent and includes a limited, non-transferable license under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. For inquiries email echelon@echelon-inc.com.