CAS Number: 124485-41-2
Molecular Weight: 717.75
Salt Form: TFA
Sequence (3-letter): Z-Val-Val-Arg-AFC
Sequence (1-letter): Z-VVR-AFC
Storage: -20 °C or below
Z-Val-Val-Arg-AFC is a fluorogenic substrate for cathepsin S, a lysosomal cysteine protease with important roles in MHC Class II antigen presentation and nociception. Cathepsin S also cleaves several extracellular matrix proteins (laminin, fibronectin elastin, osteocalcin) and glycosaminoglycans (heparin sulfate, chondroitin sulfate).
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.