CAS Number: 65147-22-0
Molecular Weight: 666.24
Salt Form: TFA
Sequence (3-letter): Z-Phe-Arg-AFC
Sequence (1-letter): Z-FR-AFC
Storage: -20 °C or below
Z-Phe-Arg-AFC is a fluorogenic for Cathepsin L. Cathepsin L is a lysosomal cysteine protease with an important role in initiating protein degradation. Cathepsin L is also upregulated in several cancers and has been suggested as a drug target. In addition, extracellular secretion of Cathepsin L by tumors implicates it as a potential serum biomarker for cancer progression.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.