Z-Arg-Arg-AMC (Cathepsin B substrate)

CAS Number: 88937-61-5
Molecular Weight: 621.3
Salt Form: HCl
Purity: >96%
Sequence (3-letter): Z-Arg-Arg-AMC
Sequence (1-letter): Z-RR-AMC
Storage: -20 °C or below

Z-RR-AMC is a fluorogenic substrate for Cathepsin B. Cathepsin B is a lysosomal cysteine protease with a role in intracellular protein degradation. Cathepsin B crosses the blood-brain barrier and elevated levels are implicated in many neurological maladies including traumatic brain injury, epilepsy, and Alzheimer’s disease.

Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.

References

1) Hulkower KI, Butler CC, Linebaugh BE, Klaus JL, Keppler D, Giranda VL, Sloane BF. Fluorescent microplate assay for cancer cell-associated cathepsin B. Eur J Biochem. 2000 Jul;267(13):4165-70. doi: 10.1046/j.1432-1327.2000.01458.x