Molecular Weight: 620.54
Salt Form: N/A
Sequence (3-letter): Z-Ala-Ala-Asp-AFC
Sequence (1-letter): (Z)AAD(AFC)
Storage: -20 °C or below
Z-Ala-Ala-Asp-AFC is a fluorogenic substrate for cathepsin S, a lysosomal cysteine protease with important roles in MHC Class II antigen presentation and nociception. Cathepsin S also cleaves several extracellular matrix proteins (laminin, fibronectin elastin, osteocalcin) and glycosaminoglycans (heparin sulfate, chondroitin sulfate).
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.