Unlabeled Shuttle PIP Carrier 2 (Histone H1) has successfully delivered the following phosphoinositides into cells: PtdIns(4,5)P2, PtdIns(3,4)P2, PtdIns(3,5)P2, PtdIns(3,4,5)P3, and their fluorescent analogs.
To provide our customers with additional options in designing their own cell-delivery applications, Echelon offers fluorescent and non-fluorescent carriers as stand alone products outside our Shuttle PIP kits. Customers can use the most effective carrier for their system along with the individual fluorescent or non-fluorescent phosphoinositides of their choice.
Provided as 2 x 50 nmoles
Storage
Store dried product at -20 °C. Once reconstituted, store at 2-8 °C. Do not freeze thaw multiple times. Note: phosphate buffers are not recommended and may alter complex formation with phosphoinositides. We do not recommend storing carriers and PIPs together as complexes.
Publications
Powered by Bioz1) Maffucci, T., A. Brancaccio, et al. (2003). “Insulin induces phosphatidylinositol-3-phosphate formation through TC10 activation.” Embo J 22(16): 4178-89.
2) Soughayer, J. S., Y. Wang, et al. (2004). “Characterization of TAT-mediated transport of detachable kinase substrates.” Biochemistry 43(26): 8528-40.
3) Im, E., S. H. Rhee, et al. (2010). “Corticotropin-releasing hormone family of peptides regulates intestinal angiogenesis.” Gastroenterology 138(7): 2457-67
