TMR Labeled Shuttle PIP Carrier 2 (Histone H1) is labeled with a red dye (tetramethylrhodamine) and has successfully delivered the following phosphoinositides into cells: PtdIns(4,5)P2, PtdIns(3,4)P2, PtdIns(3,5)P2, PtdIns(3,4,5)P3, and their fluorescent analogs.
To provide our customers with additional options in designing their own cell-delivery applications, Echelon offers fluorescent and non-fluorescent carriers as stand alone products outside our Shuttle PIP kits. Customers can use the most effective carrier for their system along with the individual fluorescent or non-fluorescent phosphoinositides of their choice.
Provided as 2×10 nmoles
Protect from moisture and light and store at -20 °C until reconstituted. Reconstitute with water or other aqueous solutions and store at 4 °C in the dark after reconstituting for up to 3 months. Multiple freeze thawing is not recommended. Note: phosphate buffers are not recommended and may alter complex formation with phosphoinositides. We do not recommend storing carriers and PIPs together as complexes.
1) Ozaki, S., D. B. DeWald, et al. (2000). “Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers.” Proc Natl Acad Sci U S A 97(21): 11286-11291.
2) Dewald, D. B., S. Ozaki, et al. (2005). “Cellular calcium mobilization in response to phosphoinositide delivery.” Cell Calcium 38(2): 59-72.