Sphingosine Beads

Product Number: S-6100

Support

Lipid coated beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein, proteins in cell lysate, or binding of radiolabeled in vitro translation products.

Sphingosine Beads are composed of agarose with 10 nanomoles of bound Sphingosine per mL of beads, enough for several protein binding experiments. Each 1 mL order includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

4 °C – do not freeze

Categories	Biochemical Reagents
Filter	Beads, Sphingolipid, Sphingosine

Technical Data Sheet

Blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Blog: Identifying lipid-protein interactions with Lipid Beads and LC/MS

1) Alvarez, S. E., et al. (2010). “Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2.” Nature 465(7301): 1084.
2) Takasugi, N., et al. (2011). “BACE1 activity is modulated by cell-associated sphingosine-1-phosphate.” J Neurosci 31(18): 6850-6857
3) Parham, K. A., et al. (2015). “Sphingosine 1-phosphate is a ligand for peroxisome proliferator-activated receptor-γ that regulates neoangiogenesis.” The FASEB Journal 29(9): 3638-3653.
4) Nair, S., et al. (2018). “Antigen-mediated regulation in monoclonal gammopathies and myeloma.” JCI insight 3(8) e98259.