PI(3,5)P2 Grip (G-3501) is a recombinant N-terminal GST fusion PI(3,5)P2 binding protein expressed in E. Coli. G-3501 has been validated for use in ELISA and as a control for lipid pulldown experiments.
Phosphatidylinositol 3,5-bisphosphate is in very low abundance and is required for retrograde membrane trafficking from lysosomal and late endosomal compartments to the Golgi and is involved in autophagy. PI(3,5)P2 levels are controlled by the 5-kinase PIKfyve and the 5-phosphatase Fig4/Sac3 and disregulation is linked to several human neuropathies (eg Chacot-Marie-Tooth disease).
Storage: -20 °C. Aliquot and freeze if intended for multiple uses. Protein will be stable for at least 6 months from date of shipment.
1. Dove S.K., Piper, R.C., McEwen, R.K., Yu, J.W., King, M.C., Hughes, D.C., Thuring, J., Holmes, A.B., Cooke, F.T., Michell, R.H., Parker, P.J., & Lemmon,
M.A. (2004) Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors. EMBO J. 1-12.
2. Dong X., Shen D., Wang X., Dawson T., Li X., Zhang Q., Cheng X., Zhang Y., Weisman L.S., Delling M. & Xu H. (2010) PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome. Nat Comm 1(38)