Purified Anti-PtdIns(3,4,5)P3 monoclonal antibody conjugated with fluorescein.
Applications: ELISA, Immunofluorescence
Product Background:Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2.
Protect from light. 2-8 °C for up to 30 days, -20 °C for greater than 30 days. Do not freeze thaw multiple times.
Featured in Publications
1) Vedham, V., H. Phee, et al. (2005). “Vav activation and function as a rac guanine nucleotide exchange factor in macrophage colony-stimulating factor-induced macrophage chemotaxis.” Mol Cell Biol 25(10): 4211-20.
2) Gupta, S., A. R. Ramjaun, et al. (2007). “Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice.” Cell 129(5): 957-68.
3) Lin, H. V., L. Plum, et al. (2010). “Divergent Regulation of Energy Expenditure and Hepatic Glucose Production by Insulin Receptor in Agouti-Related Protein and POMC Neurons.” Diabetes 59(2): 337-346.
Product keywords: PIP3, PI(3,4,5)P3, PtdIns(3,4,5)P3, IgM, anti-PIP3