PolyPIPosomes are polymerized liposome-like nanoparticles (~200 nm) which show markedly increased stability (up to 6 months) vs. conventional liposomes. A biotin tag is incorporated for easy detection of PIPn-binding proteins with streptavidin reagents or coated surfaces.
PolyPIPosomes have demonstrated utility in a number of assay formats:
- Protein pull-down
- Surface Plasmon Resonance
- Liposome overlay assay
PI PolyPIPosomes are provided as a 1mM total lipid solution containing 5% phosphatidylinositol (PI). Each order of 250 μL or 1 mL of PolyPIPosomes includes 100 μL of Control PolyPIPosomes.
Featured in Publications
1) Ferguson, C. G., R. D. James, et al. (2005). “Phosphoinositide-Containing Polymerized Liposomes: Stable Membrane-Mimetic Vesicles for Protein-Lipid Binding Analysis.” Bioconjug Chem 16(6): 1475-1483.
2) Elkin, S. K., D. Ivanov, et al. (2005). “A PHD finger motif in the C-terminus of RAG2 modulates recombination activity.” J Biol Chem 280(31): 28701-10.
3) Mustafa, A. K., D. B. van Rossum, et al. (2009). “Glutamatergic regulation of serine racemase via reversal of PIP2 inhibition.” Proc Natl Acad Sci U S A 106(8): 2921-6.
4) Zemskov, E. A., I. Mikhailenko, et al. (2011). “Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.” PLoS One 6(4): e19414.
5) Tan, X., Y. Sun, et al. (2015). “LAPTM4B is a PtdIns(4,5)P2 effector that regulates EGFR signaling, lysosomal sorting, and degradation.” Embo J 34(4): 475-90.
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