Recombinant anti-p53 Nanobody, Clone # Nb139, COOH-terminal HA tag.
Type: VHH single domain
Source: E. Coli
Applications: ELISA, Immunoprecipitation, Pull down
Clonality: Monoclonal
Background: p53 DBD VHH Antibody was produced in an alpaca with human recombinant p53 DNA-binding domain then cloned for recombinant production in E. Coli. p53 is a tetrameric transcription factor and tumor suppressor that controls numerous aspects of cellular behavior. Mutations in p53 are observed in over 50% of human cancers with several hot spot mutations occurring in the DNA binding domain of p53.
Recombinant anti-p53 Nanobody 139 was developed by Gulliver Biomed.
Storage
Store at -20°C upon arrival. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
Publications
1) Bethuyne J, et al. (2014) A nanobody modulates the p53 transcriptional program without perturbing its functional architechture. Nucleic Acids Res; 42(20):12928-12938
2) Steels, A et al. (2018) Intracellular displacement of p53 using transactivation domain (p53 TAD) specific nanobodies. MAbs; 10(7):1045-1059
3) Steels, A et al. (2019) Nb-induced stabilisation of p53 in HPV-infected cells. Sci Rep; 9(1):12680
Keywords: p53, DNA-binding domain, DBD, nanobody, VHH, antibody, single domain
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