Purified Anti-PtdIns(4)P monoclonal antibody.
Applications: ELISA, Immunofluorescence, Fluorescence Polarization, Protein-Lipid Overlay (PIP Strip)
Product Background: Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4-phosphate (PI(4)P) is the biosynthetic precursor to PI(4,5)P2 and has an important roles in regulating sphingomyelin and glycosphingolipid metabolism and membrane trafficking at the exit of the Golgi complex.
2-8 °C for up to 30 days, -20 °C for greater than 30 days. Do not freeze thaw multiple times.
Featured in Publications
1) Weber, S. S., C. Ragaz, et al. (2006). “Legionella pneumophila exploits PI(4)P to anchor secreted effector proteins to the replicative vacuole.” PLoS Pathog 2(5): e46.
2) Blagoveshchenskaya, A., F. Y. Cheong, et al. (2008). “Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1.” J Cell Biol 180(4): 803-12.
3) Cheong, F. Y., V. Sharma, et al. (2010). “Spatial Regulation of Golgi Phosphatidylinositol-4-Phosphate is Required for Enzyme Localization and Glycosylation Fidelity.” Traffic 11(9): 1180-1190.
4) Bojjireddy, N., J. Botyanszki, et al. “Pharmacological and Genetic Targeting of the PI4KA Enzyme Reveals Its Important Role in Maintaining Plasma Membrane Phosphatidylinositol 4-Phosphate and Phosphatidylinositol 4,5-Bisphosphate Levels.” Journal of Biological Chemistry 289(9): 6120-6132.
Product keywords: PtdIns(4)P, anti-PI(4)P, PI4P, IgM