CAS Number: 70967-90-7
Molecular Weight: 590.27
Salt Form: N/A
Sequence (3-letter): MeOSuc-Ala-Ala-Pro-Val-pNA
Sequence (1-letter): MeOSuc-AAPV-pNA
Storage: -20 °C or below
Solubility: DMSO (20 mM), methanol (1 mg/ml), DMF, and ethanol
Pancreatic Leukocyte Elastase Substrate (MeOSuc-AAPV-pNA), aka Elastase Substrate 1, is a highly sensitive colorimetric substrate for pancreatic leukocyte elastase (neutrophil elastase). Pancreatic leukocyte elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy bacteria as well as host tissue. MeOSuc-AAPV-pNA can be used for in vitro assays with purified enzyme or with biological fluids or conditioned medium. Reaction progress is detected by monitoring substrate hydrolysis at 405-410 nm.
This product is not a substrate for cathepsin G or chymotrypsin.
1. A.M. et al. (2002) “Dipeptidyl peptidase I activates neutrophil-derived serine proteases and regulates the development of acute experimental arthritis” J. Clin. Invest. 109 (3): 363–371.
2. Lonky, S. A and Whole, H. (1983) “Regulation of elastolysis of insoluble elastin by human leukocyte elastase: stimulation by lysine rich lugans, anionic detergents and ionic strength” Biochemistry 22: 3714.
3. Stein, R. L., Viscarello, B. R., and Vildonger, R. A. (1984) “Catalysis by human leukocyte elastase. II. Rate limiting deacylation for specific p-nitroanilides and amides” J. Am. Chem. Soc.. 106: 796.