Membrane Lipid Arrays

Membrane Lipid Arrays are 4 x 5 cm hydrophobic membranes that have been spotted with a concentration gradient of eight different biologically important lipids found in cell membranes. They are used to determine the relative degree of binding of your protein of interest towards one or more of these lipids in a simple protein lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.

Membrane Lipid Arrays have 100, 50, 25, 12.5, 6.25, 3.13, and 1.56 pmol of the following lipids:

Diacylglycerol (DAG)
Phosphatidic acid (PA)
Phosphatidylserine (PS)
Phosphatidylinositol (PtdIns)
Phosphatidylethanolamine (PE)
Phosphatidylcholine (PC)
Phosphatidylglycerol (PG)
Sphingomyelin

Storage

2-8 °C. Product is moisture and light sensitive

Publications

Powered by Bioz See more details on Bioz

1) Dibble, C. F., J. A. Horst, et al. (2010). “Defining the functional domain of programmed cell death 10 through its interactions with phosphatidylinositol-3,4,5-trisphosphate.” PLoS One 5(7): e11740.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

Documents

Technical Data Sheet

Protocol

FAQ