CAS Number: 189696-01-3
Molecular Weight: 1144.43
Salt Form: N/A
Sequence (3-letter): Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(DNP)-OH
Sequence (1-letter): (Mca)YVADAP(Lys(DNP))-OH
Storage: -20 °C or below
Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(DNP)-OH is a FRET substrate for caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that preferentially cleaves substrates built on VAD (val-ala-asp) or WEAD (trp-glu-ala-asp) sequences. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cells.
Activity can be continuously measured by excitation at 325nm and emission at 392 nm