Hyaluronidase Activity ELISA

Product Number: K-6000


The Hyaluronidase Activity ELISA is a quantitative immunoassay designed for in vitro measurement of hyaluronidase activity in biological samples.

Sample Type: Biological samples (human sera, plasma, and urine samples) and purified Hyaluronidase.
Sample Volume: 12 µL/ duplicates
Assay Incubation Time: 2 hours and 30 min + enzyme reaction time
Assay Range: 0.078-5 µg HA

Product Background
The Hyaluronidase Activity ELISA is a quantitative immunoassay designed for the in vitro measurement of hyaluronidase activity in biological samples.

Hyaluronidase reactions are performed in a 96-well plate pre-coated with HA substrate. The activity of the hyaluronidase is determined by comparing HA substrate levels post reaction to a standard curve of pre-coated HA substrate. The Hyaluronidase Activity ELISA has been validated with human sera, plasma, urine and purified hyaluronidase from bovine testes. The Hyaluronidase Activity ELISA provides a robust and simple method for researchers to measure hyaluronidase activity in biological samples.

Hyaluronidases are a group of enzymes that degrade Hyaluronan (HA), a linear polysaccharide comprised of a repeating disaccharide of N-acetylglucosamine and D-glucuronic acid. HA is involved in many biological processes including structural support, cell migration and tissue turnover. Circulating HA levels correlate in several disorders such as liver disease and certain types of cancer. Hyaluronidases have become an important area of study due to their regulatory function in HA metabolism and their role in physiological processes such as fertilization and wound healing. Elevated expression levels of the gene encoding Hyaluronidase 1 have been found in atypical ductal hyperplastic tissues, suggesting the possibility that it may be a viable biomarker for identifying premalignant lesions that can develop into breast cancer. Research has also shown that hyaluronidases are involved in several pathological processes such as bacterial pathogenesis, the spreading of toxins/venoms and cancer progression, making hyaluronidases a potential pharmacological target.

Product Keywords: Hyaluronan, Hyaluronate, Liver Cirrhosis, Rheumatoid Arthritis, Osteoarthritis, Liver Fibrosis


Kits & Assays


Activity, ECM, ELISA, Hyaluronic Acid

Shipping Temp

Dry ice or gel ice depending on destination


-20 °C

Technical Data Sheet

1. Chang, S.-H., et al. (2017). “Transforming growth factor-β-mediated CD44/STAT3 signaling contributes to the development of atrial fibrosis and fibrillation.” Basic Research in Cardiology 112(5): 58.
2. Margraf, A., et al. (2018). “6% Hydroxyethyl starch (HES 130/0.4) diminishes glycocalyx degradation and decreases vascular permeability during systemic and pulmonary inflammation in mice.” Critical Care 22(1): 111.
3. De Robertis, M., et al. (2018). “In Vivo Evaluation of a New Recombinant Hyaluronidase to Improve Gene Electro-Transfer Protocols for DNA-Based Drug Delivery against Cancer.” Cancers 10(11): 405.
4. Kim, S., et al. (2020). “Intrastromal Injection of Hyaluronidase Alters the Structural and Biomechanical Properties of the Corneal Stroma.” Translational Vision Science & Technology 9(6): 21-21.
5. Witschen, P. M., et al. (2023). “Characterization of Hyaluronan Localization in the Developing Mammary Gland and Mammary Tumors.” Journal of Mammary Gland Biology and Neoplasia 28(1): 1.
6. Kim, J.-S., et al. (2023). “ECM-targeting bacteria enhance chemotherapeutic drug efficacy by lowering IFP in tumor mouse models.” Journal of Controlled Release 355: 199-210.
7. Xing, H., et al. (2023). “HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice.” Renal Failure 45(1): 2188966.

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