The Hyaluronidase Activity ELISA is a quantitative immunoassay designed for in vitro measurement of hyaluronidase activity in biological samples.
Sample Type: Biological samples (human sera, plasma, and urine samples) and purified Hyaluronidase.
Sample Volume: 12 µL/ duplicates
Assay Incubation Time: 2 hours and 30 min + enzyme reaction time
Assay Range: 0.078-5 µg HA
Product Background
Powered by BiozThe Hyaluronidase Activity ELISA is a quantitative immunoassay for measuring hyaluronidase activity in biological samples. This in vitro test uses a 96-well plate pre-coated with hyaluronan (HA) substrate. The activity of hyaluronidase is determined by comparing the remaining HA substrate, after the reaction, to a standard curve of pre-coated HA.
Validated for use with human serum, plasma, urine, and purified bovine testes hyaluronidase, the Hyaluronidase Activity ELISA offers a simple, reliable method for detecting hyaluronidase activity in various biological samples.
Hyaluronidases are enzymes that break down hyaluronan (HA), a linear polysaccharide composed of N-acetylglucosamine and D-glucuronic acid. HA plays a vital role in various biological processes, including structural support, cell migration, and tissue repair. Elevated HA levels are often observed in conditions like liver disease and certain cancers.
The study of hyaluronidases has gained importance due to their regulatory function in HA metabolism and their involvement in critical physiological processes such as fertilization and wound healing. For example, the gene encoding Hyaluronidase 1 has been found to be overexpressed in atypical ductal hyperplastic tissues, highlighting its potential as a biomarker for identifying precancerous breast lesions.
Moreover, hyaluronidases are implicated in several pathological conditions, including bacterial infections, toxin/venom spread, and cancer metastasis. This makes hyaluronidases a promising pharmacological target for therapeutic intervention.
Product Keywords: Hyaluronan, Hyaluronate, Liver Cirrhosis, Rheumatoid Arthritis, Osteoarthritis, Liver Fibrosis
