Hyaluronic Acid Sandwich ELISA

Product Number: K-4800


The Hyaluronic Acid Sandwich ELISA offers a simple, effective method for determining Hyaluronic Acid (HA) levels in human and animal biological fluids or cell supernatants.

Assay Range: 12.5-3200 ng/ml
Sensitivity: 12.5 ng/mL
Sample Volume: 25 µL/ duplicate
Sample Considerations: Measures HA 130 kDa

The Hyaluronic Acid Sandwich ELISA is a 96-well, quantitative immunoassay designed for in vitro measurement of HA levels in cell culture supernatant, or human/animal biological fluids.

The concentration of HA in the sample is determined using a standard curve of known amounts of HA. The HA Sandwich ELISA was validated with human sera samples. Only a small amount of sample (25 µL) is needed for running duplicates measurements.

The HA Sandwich ELISA provides a robust and simple method for researchers to measure HA in biological samples.

For help on selecting the correct HA detection assay see the HA Assay Comparison Chart. Also, check out this article on “How to select Hyaluronic Acid (HA) quantitative tests”.

Product Keywords: Hyaluronan, Hyaluronate, Hyaluronic Acid, Liver Cirrhosis, Rheumatoid Arthritis, Osteoarthritis, Liver Fibrosis


HA Assay Comparison Chart

Blog: Which Hyaluronic Acid Assay Should I Use?


Kits & Assays


ELISA, Hyaluronic Acid

Shipping Temp

Dry ice or gel ice depending on destination


-20 °C

1) D. Bruegger, F. Brettner, I. Rossberg, C. Nussbaum, C. Kowalski, K. Januszewska, B. F. Becker and D. Chappell. (2015) “Acute Degradation of the Endothelial Glycocalyx in Infants Undergoing Cardiac Surgical Procedures” The Annals of Thoracic Surgery 99(3), 926–931 doi: 10.1016/j.athoracsur.2014.10.013
2) J. Qin, J. Kilkus and G. Dawson. (2015) “The hyaluronic acid inhibitor 4-methylumbelliforone is an NSMase2 activator-A novel antitumor mechanism of MU” Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids 1861(2), 78-90 doi:10.1016/j.bbalip.2015.11.001

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