The HA AlpahScreen Assay is a competitive, laser-excited, bead-based assay. The HA present in your sample competes with the Biotin-HA conjugated on donor beads for binding to the His-tag HA Detector conjugated on acceptor beads. Signal is generated when the donor and acceptor beads are within 200 nm. Therefore, when the Biotin-HA/HA Detector interaction is interrupted by the HA present in your sample, signal is no longer generated. The laser-excited, bead-based assay significantly increases assay sensitivity and reduces assay time to just 90 minutes by eliminating the washing steps. This assay can pick up all sizes of HA with concentrations as low as 20 ng/mL using just 5 µL of sample.
Best For: Samples that contain large MW range of HA at low concentration with limited sample volume